Post-Diploma Certificate in Molecular Biotechnology under the Advanced Diploma in Medical and Pharmaceutical Technology (ADMPT)

  

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Post-Diploma Certificate in Cell and Molecular Biology Module Synopsis Cell Biology This module explores mammalian cells and tissue systems. Topics include the fundamental chemicals of life, structure and function of cells and organelles, cellular transport systems, cell division and DNA replication, transcription and translation. Laboratory classes will support lecture materials. Molecular Biology This module highlights prokaryotic and eukaryotic genetics at the molecular level. Topics include protein synthesis, regulation of gene expression in microorganisms, gene mutation, DNA repair mechanisms, and applications of gene manipulations in microorganisms. Also covered are generation and isolation of target DNA fragment, insertion of target DNA into vectors, transformation of E.coli competent cells, identification of recombinant clones, protein expression of target DNA, and the concept of how cells undergo apoptosis or become tumorous. Laboratory classes will provide participants with skills in DNA extraction, cloning, DNA fingerprinting, PCR analysis, southern blot analysis, western blot analysis, and protein expression and purification. Applied Genomics And Proteomics This module provides an insight into the fields of genomics and proteomics. Participants will study the functions of genes and their interaction within a genome, the importance of human genome project in the discovery of new drugs, genetically modified organisms, protein expression, structure and functions of proteins, methods of purification and analyses of proteins. Mammalian Cell Culture This module equips participants with strong aseptic techniques and an understanding of the application of cell culture in biopharmaceutical production and research and development. Topics include types of sterilization methods, preparation of media, maintenance of cell cultures and the use of bioreactors in culturing cells.

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